GeneMarker® HID is an excellent choice for all forensic profiling applications. This software can be employed as a "biologist friendly" replacement for GeneScan®/GenoTyper® or as an alternative to GeneMapper® ID. GeneMarker® HID uses advanced Windows® technology that is intuitive and easy to learn. In addition to standard STR analysis, GeneMarker HID has been optimized to analyze profiles from YSTRs, LCN samples, ABI's MiniFilerTM Kit, and Locus Specific Bracket (LSB) data. GeneMarker HID includes applications to assist experts with Mixture Analysis. Relationship testing and databasing capabilities which are essential for crime scene investigation, missing persons identification and paternity testing.
GeneMarker®HID Provides:
Reduce casework backlog with these key software features:
Accurate
Advantages of GeneMarker HID are ease of use and accurate, quick analysis.
GeneMarker HID utilizes unique sizing algorithms and pattern recognition
technology to provide unparalleled accuracy up to 99%.
The majority of functions have been completely automated, using a “smart”
expert system software approach to eliminate the need for constant analyst intervention,
and to improve analysis to analysis consistency.
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Append Projects Quickly and Consistently
Often data pertinent to the case becomes available after the initial project analysis. In just three ‘clicks’ the new files are added and processed using the same parameters as the original project. This append feature provides accurate, consistent updates to projects.
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Rapid Data
Processing, Instrument Correction and Compatibility
GeneMarker HID can perform STR analysis on over 1,000 lanes of data using
the following ABI
and Promega kits:
- Identifiler®
- SGM Plus®
- PowerPlex®16
- And Many More!
The software accepts data format output by ABI PRISM® 310/3100/3130/3730
Genetic Analyzer instruments, as well as MegaBACE and Beckman capillary electrophoresis systems.
GeneMarker HID automatically adjusts for— instrument spike, color
pull-up, peak pull-up, noisy data, saturated peaks, and stutter peaks—saving
significant analysis time and cost, efficiently analyzing raw STR data
within seconds. In addition, GeneMarker HID allows the user to conduct
a lane-by-lane overview of unprocessed raw data, permitting a quick quality
control assessment of your profiles.
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Three Simple
Clicks to Analysis
Setting analysis parameters is accomplished through 3
easy-to-use dialog boxes. Once parameters have been set, they can be
saved for future analysis, allowing “single click” operation
of the AutoRun feature.
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Enhanced Quality
Assessment
A few notable features of GeneMarker HID’s analysis window include automatic RFU intensity ranging in the electropherogram, distinct peak flags, and several customizable viewing modes. In addition to a user friendly interface, GeneMarker HID also provides an enhanced quality scoring system that flags suspect alleles and identifies samples that have questionable size calling results.
Analysis Window Customizing Options
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Flagging Off-Ladder Alleles
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All Color Browser |
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Customizable
Panels and Size Standards
Panel templates and size standards can either be selected from several
embedded templates or the user can create and save custom templates. Previously
saved ABI panels can also be imported from GeneMapper. Raw data and size
calling parameters are also user-definable and once the parameters have
been set, they can be saved and called back for future analysis.
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Quality Analysis of Size Call
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Mixture Analysis
Short Tandem Repeat (STR) genotyping is instrumental in data analysis for a wide range of human identification applications. Very often samples from mass disasters and crime scene investigations are mixtures rather than single source; requiring additional analysis after the allele calls are made. GeneMarker HID has all of the features of an expert system for consistent and accurate genotype determination and an embedded mixture analysis application; automating repetitive calculations and providing expert assistance to the analyst. The mixture analysis application was developed using recommendations of the DNA commission of the International Society of Forensic Genetics (Gill et al., 2006) and methods of Clayton et al., 1998 and Gill et al., 1998
Potential Mixture Samples Identified Automatically in Main Analysis Screen
Complete Mixture Analysis Application with or without Reference Samples
- Analysis Parameters easily set in dialog box
- Groups files by number of contributors
- Identifies contributor single source files
- Displays all possible allele combinations for contributors, color coding those that meet all mixture analysis parameters
- Determines major and minor contributors
- Searches database for exact match or potential near relatives
Calculates:
- Major Mx ratio, Residual, Heterozygous Imbalance for each combination of alleles in two contributor mixtures
- Likelihood Ratios at each marker and combined LR in two contributor mixtures
- Probability of Inclusion and Probability of Exclusion for any mixture sample (two contributors or three or more)
Mixture Analysis with Two Single Source Reference Files
Identifies Potential Suspect Profile without Suspect Reference Profile
Search database using major contributor potential suspect from deconvolution of mixture sample
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Relationship Testing Tools
GeneMarker HID includes an imbedded Pedigree Tool for family tree analysis. This function allows the user to easily add mates and offspring and it automatically identifies allele conflicts between parents and/or siblings. Create an original pedigree file from new data or import pre-made pedigree files (.pre, .ped, .smp).
Additional applications include relationship testing and databasing of family samples. See our Relationship Testing poster from the 2007 International Symposium on Human Identification.
Pedigree
Analysis
An expanding pedigree field accommodates and saves multiple children and mates, multiple generations and phenotype information. The pedigree files are compatible with Bennet‘s Standards for human pedigree analysis. The phenotype information and relationships are saved in files with unlimited capacity.
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Allele Conflicts Linked to Electropherograms
Family Group tool automatically draws paternity trios. Allele conflicts are linked to the electropherograms for immediate identification in paternity tests.
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Missing Persons Application:
Locates Duplicate Samples and Nearest Relatives from DataBase Search
Robust statistical analysis calculates combined Likelihood Ratios (using identity by descent, Brenner 1997)
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Kinship Analysis:
Likelihood Ratios, Probabilities and Relationship Level Options
Kinship Analysis options provide flexibility to analyze data for casework and legal presentation. Options include Relationship levels, Likelihood Ratios and/or probabilities for each locus and combined values.
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Easily Compare Analyses
Select to previously analyzed projects and compare the results with GeneMarker HID's unique Project Comparison Tool. The Project Comparison Tool aligns two projects and identifies differences in peak size, height, and allele call. Additionally, if an analyst edited an allele call, the results can be immediately corroborated. The Project Comparison Tool can also be used during validation to examine the affects of certain analysis parameters on allele calling.
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Supervisory Tools
Select two previously analyzed projects and compare the results with GeneMarker HID's unique Project Comparison Tool. The Project Comparison Tool aligns two projects and identifies differences in peak size, height, and allele call. Additionally, if an analyst edited an allele call, the results can be immediately corroborated. The Project Comparison Tool can also be used during validation to examine the affects of certain analysis parameters on allele calling.
Rapid Analysis Review
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Provides an Audit Trail
An Audit Trail is available when ‘Record Data Edit History’ is selected in the User Manager Settings. Any change to allele calls in the analysis will be recorded. Changes can also be recovered in the Edit History feature.
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Reduces Analyst Fatigue
Design features that reduce analyst fatigue:
- Auto-scaling feature
- Linked electropherogram to allele report
- Report format options
o Show only uncertain alleles
o Hide duplicate sample names
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Numerous Printing
& Reporting Options
Nearly every function of GeneMarker HID has been automated
so that once the template is selected from the menu, or created by the
user, the software automatically performs the initial data analysis in
preparation for user review, thereby providing a myriad of display and
reporting options. Analysis results can be sorted and manipulated to meet
report requirements and then saved as a text file for input into LIMS
or other data base systems. CODIS files can be easily generated and exported.
In addition, GeneMarker HID will print electropherograms and peak tables
in bulk for either all or selected sample sets in a logical, easy to read
format.
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Student/Instructor Version
Practical data analysis with the same software used in Casework and CODIS laboratories is available to instructional programs of any size.
The GeneMarkerHID instructional package includes:
- One perpetual license of GeneMarker® HID software with annual update
- Pre-set forensic chemistry Panels including ABI’s Identifiler® and Yfiler® kits and Promega’s PowerPlex® kits
- Instruction manual
- NIST dataset for casework analysis (mixed source) and anonymous single source data
- Unlimited access to SoftGenetics forensic analysis technical support
- 120-day time-limited, fully-functional student licenses of GeneMarker® HID
The combination of the datasets and the fully functional program allow students to gain hands on experience in DNA casework analysis, without the high costs of maintaining genetic analyzers and associated laboratory equipment.
ORDER FORM
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GeneMarker HID Missing Persons Application Note [PDF]
GeneMarker HID Crime Scene Suspect Application Note [PDF]
GeneMarker HID Paternity Testing Application Note [PDF]
If you would like to download the GeneMarker® HID brochure please click here. (Adobe
PDF 630kb)
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